HEMAGGLUTINATION REACTION BY JAPANESE B ENCEPHALITIS VIRUS

Key words: ELISA IgM titer, Hemagglutination inhibition, Japanese encephalitis virus antibodies, Japanese encephalitis epidemics in pigs

特 定飼 育 ブ タ10頭 につ いて,個 々 に血 液 を採取 し抗 体価 を測 定 した ところ,流 行前 期 にはELISAIgM 抗 体価 は103以 下で,HI抗 体 は検 出 され なか った.HI法 に よ るIgM抗 体 が現 われ始 め ると,ELISAIgM 抗体価 は104以 上 を示 した.ELISAIgM抗 体 は約2週 間 継続 して検 出 され た.そ の後ELISAIgM抗 体 価 は103前 後 に低 下 した が,HI反 応 は陽性 で あ り,ほ とん どIgG抗 体 であ った. と畜場 ブタ につ い ては,定 期 的 に20頭 ず つ検査 し,非 流 行期 及 び流行前 期 のELISAIgM抗 体 価 は103 以下 で あ った.流 行期 に入 る とHI法 に よるIgM抗 体 は陽性 とな り,そ の ほ とん どがELISAIgM...

Binary Ethylenimine Inactivated Japanese Encephalitis Virus Antigen Reveals Hemagglutination

Severe climate change and global warming may impact significantly on vector-borne disease including Japanese encephalitis (JE) infection in human and animals. Thus, veterinary authority requires large quantity of diagnostic tools to survey vector-borne diseases. New producing method having a relation with JE antigen is needed to substitute conventional sucrose-acetone extraction method using su...

Aerosol stability and respiratory infectivity of japanese B encephalitis virus.

Experiments were conducted to examine the aerosol stability and respiratory infectivity of Japanese B encephalitis virus. At 75 degrees F (about 24 degrees C), survival of the virus as aerosol was inversely related to relative humidity. After correction for physical decay, the mean virus half-lives of the virus were 28, 38, and 62 min at relative humiditis of 80, 55, and 30%, respectively. Viru...

Neuropathogenesis of Japanese encephalitis virus.

In the central nervous system, the Japanese encephalitis virus can replicate only in neurons. The mechanism of the type of neurotropism was analyzed. The susceptibility to Japanese encephalitis virus infection in the rat brain was closely associated with neuronal immaturity. The initial specific binding of the virus to cells is one of the reasons for neurotropism of the Japanese encephalitis vi...

Immunobiology of Japanese Encephalitis Virus